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. 2018 Mar 19;8:4768. doi: 10.1038/s41598-018-23030-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of helium on Cav-1 levels in HUVEC and HCAEC. Panel A: Western blot results of the cellular ratio of Cav-1 compared to GAPDH loading controls from different time points following helium or control gas treatment in HUVEC. Panel B: Western blot results of the supernatant ratio of Cav-1 compared to albumin loading controls from different time points following helium or control gas treatment in HUVEC. Panel C: Western blot results of the cellular ratio of Cav-1 compared to GAPDH loading controls from different time points following helium or control gas treatment in HCAEC. Panel D: Western blot results of the supernatant ratio of Cav-1 compared to albumin loading controls from different time points following helium or control gas treatment in HCAEC. Cropped images of representative Western blot results are displayed below the respective graphs. Full-length blots are presented in Supplementary Figs S5–S8. Data are represented as mean ± SD, *p < 0.05. HUVEC = human umbilical vein endothelial cells. HCAEC = human coronary artery endothelial cells.