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. 2018 Mar 19;8:4768. doi: 10.1038/s41598-018-23030-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of helium on VE-Cadherin and Cx43 levels in HUVEC and HCAEC. Panel A: Western blot results of the ratio VE-Cadherin compared to GAPDH loading controls in HUVEC at different time points following helium or control gas treatment. n = 14. Panel B: Western blot results of the ratio Cx43 compared to Tubulin loading controls levels in HUVEC at different time points following helium or control gas treatment. n = 11. Panel C: Western blot results of the ratio VE-Cadherin compared to GAPDH loading controls in HCAEC at different time points following helium or control gas treatment. n = 14. Panel D: Western blot results of the ratio of Cx43 compared to GAPDH loading controls in HCAEC at different time points following helium or control gas treatment. n = 14. Cropped images of representative Western blot results are displayed below the respective graphs. Full-length blots are presented in Supplementary Figs S9–S12. Data are represented as mean ± SD, *p < 0.05. HUVEC = human umbilical vein endothelial cells. HCAEC = human coronary artery endothelial cells.