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. 2018 Mar 19;8:4768. doi: 10.1038/s41598-018-23030-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cav-1 siRNA transfection in HUVEC. Panel A: Western blot results of the ratio of Cav-1 compared to GAPDH loading control in HUVEC transfected with siRNA for Cav-1 and negative controls. Results of the transfected cells are shown as percentage of the negative control which was set at 100%. Cropped images of representative Western blot results are displayed below the respective graph. Full-length blots are presented in Supplementary Fig. S13. Panel B: Immunofluorescent images of HUVEC transfected with a red fluorescent protein (Block IT Alexa Flour). Cells were fixed 24 hours after transfection. Images of negative control are presented in Supplementary Fig. S4.