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. 2018 Mar 19;8:4768. doi: 10.1038/s41598-018-23030-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Supernatant analysis. Panel A: Effect of incubation with supernatant of helium and control gas treated cells on HUVEC Results of permeability of a confluent monolayer of HUVEC, estimated by the transfer of FITC-BSA, in HUVEC treated with supernatant of helium treated HUVEC at different time points. Controls were treated with supernatant of control gas treated HUVEC. n = 10. Panel B: Results of nanoparticle tracking analysis of supernatant after helium and control gas treated HUVEC. Results of nanoparticle tracking analysis of supernatant after helium and control gas treated HUVEC at different time points. n = 4. Data are represented as mean ± SD for panel A, and means for panel B, *p < 0.05. HUVEC = human umbilical vein endothelial cells.