Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 19;8:4806. doi: 10.1038/s41598-018-22780-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Ixonnexin promotes plasmin generation initiated with t-PA. (A and B) Ixonnexin (0–1 μg/ml) was immobilized in 96-well plates and incubated with Glu- or Lys-plasminogen (0.5 μM), respectively. Reactions were initiated with t-PA (2.5 nM for Lys-plasminogen, or 10 nM for Glu-plasminogen), and plasmin formation was detected with chromogenic substrate S2251 (250 μM). The inset shows plasmin generation in solution. (C and D) Ixonexin (0.5 μg/ml) mediated plasmin generation in the presence of increasing concentrations of Glu- and Lys-plasminogen, respectively. (E and F) Show the transformation of the data in (C and D) as the slope A405 nm/min² and Glu- and Lys-plasminogen concentration, respectively. Km of the reaction using Glu- or Lys-plasminogen was calculated by non-linear regression, as indicated in Methods.