Figure 5.

Impact of bergamottin loaded PLGA nanoparticles functionalized with galactosamine (BM-PLGA-Ga) as nanoprimer on antitumor efficacy on MDA-MB-231 tumor. Mice were xenografted with MDA-MB-231 breast tumor model and randomized when the mean tumor volume reached 200 mm³. Mice were treated as follow: control NaCl 0.9% IV injected on days 0, 4 and 8 (orange curve; n = 4); control BM-PLGA-Ga nanoparticles alone (BM = 97 µg/kg) IV injected on days 0, 4, and 8 (black curve; n = 4); Docetaxel 20 mg/kg IV injected with glucose 5% on days 0, 4 and 8 and docetaxel on days 1, 5, 9 (red curve; n = 6); BM-PLGA-Ga nanoparticles (BM = 97 µg/kg) IV injected on days 0, 4, and 8 and docetaxel 20 mg/kg IV injected on day 1, 5 and 9 (blue curve; n = 6). All solutions were injected at 10 mL/kg. (A) Tumor growth expressed as median tumor volume. Difference between the BM-PLGA-Ga/docetaxel and docetaxel alone groups was evaluated with a 2-way ANOVA analysis and a Bonferroni post-test, *** p-value < 0.001. (B) Kaplan-Meier diagram of overall survival. The median survival was 66 days for PLGA-Ga BM treated group, compared to 48 days for the docetaxel alone group and the overall survival rate was 67% versus 0% at day 55. Arrows: injections (grey arrows: BM-PLGA-Ga nanoparticles injections and black arrows: docetaxel injections) (Reproduced with author’s permission from Nano-sized cytochrome P450 3A4 inhibitors to block hepatic metabolism of docetaxel. Paolini, M. et al. International Journal of Nanomedicine. Volume 2017:12 Pages 5537–5556. DOI: https://doi.org/10.2147/IJN.S141145).