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. 2018 Mar 13;9:457. doi: 10.3389/fmicb.2018.00457

Figure 2.

Figure 2

Interaction between CABYV and TuYV structural proteins and Eph partial domain. The yeast strain NMY51 was co-transformed with pGAD-Eph and one of the following constructs: pLexAN-CPCA, pLexAN-RT*CA, pLexAN-CPTu, pLexAN-RT*Tu, or the empty pLexAN. In parallel, yeast cells were co-transformed with the empty pGAD and one of the viral pLexAN-derived plasmids mentioned above. Three colonies were allowed to grow on a medium lacking leucine and tryptophan [-LW] before being transferred onto a selective medium deprived of leucine, tryptophan, histidine and adenine [-LWHA]. Yeast cells were grown at 28°C for 3 days on [-LW] and for 14 days on [-LWHA] media.