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. 2018 Mar 19;8:4758. doi: 10.1038/s41598-018-23097-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The fluorescence images of the binary GUVs before electroporation. (A) A schematic drawing of the three different focal planes (bottom, middle and top planes) of the GUVs, not drawn to scale. (B) Two examples of the 2:8 DPPC:DPhPC GUVs, showing different gel-phase domains. The GUVs are visualised by use of fluorescence microscopy, where the dark parts represent the gel-phase domains, and the bright parts the fluid-phase or mixed domains. (C) The two different categories of the 8:2 DPPC:DPhPC GUVs. Left: a homogeneous-GUV, showing no visible domains. Right: a domain-GUV, showing a uniform distribution of dark gel-phase domains on the surface of the GUV. The scale bar is 10 μm.