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. 2018 Mar 19;9(4):423. doi: 10.1038/s41419-018-0462-8

Fig. 3. PIWIL2 inhibits the ubiquitination and degradation of HDAC3 by preventing Siah2 from binding to HDAC3.

Fig. 3

a MG132-treatment recovers HDAC3 protein level reduced by shPIWIL2. Cells were transfected with shPIWIL2 for 48 h and then treated with MG132 (10 μM) or DMSO (negative control) for 6 h. b PIWIL2 knockdown enhances the ubiquitin-mediated degradation of HDAC3. Hela cells were transfected with HA-ubiquitin vector, followed by treatment of MG132 for 6 h. Cell lysates were subjected to anti-HDAC3 antibody for WB. The “ *” indicates a non-specific bind. c PIWIL2 reduces Siah2-mediated degradation of HDAC3. Cells were transfected with PIWIL2 alone or in combination with Siah2, followed by a treatment with MG132 or DMSO as control for 6 h. d, e PIWIL2 reduces the interaction between Siah2 and HDAC3. Cells were transfected with PIWIL2 vector or PIWIL2 shRNA vector, and then analyzed by co-immuoprecipitation assays