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. 2018 Mar 19;8:4804. doi: 10.1038/s41598-018-22931-4

Figure 1.

Figure 1

CLIP is not displayed by HLA-DP molecules encoding the DP84GGPM87 phenotype regardless of HLA-DM expression on K562 cells. Surface class II, CLIP and ΔNGFR expression, along with intracellular Ii and HLA-DM expression, were analyzed on K562 transfected cells by flow cytometry following staining with specific mAbs. K562 cells were retrovirally transduced with DPA1*01:03 (DPA1) and either wild-type or mutated DPB1*04:01 (DPB4), with substitution mutations of the 84–87 region of the DPβ chain made as indicated to generate DP4 and DP484DEAV87 respectively.