Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 19;8:4804. doi: 10.1038/s41598-018-22931-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Truncation of N-terminal non-CLIP Ii region(s) restores CLIP-mediated Ii-DP association with DP4. (a) Schema depicting Ii C-terminal (Ii^ATG-120) and N-terminal (Ii^97-Stop) truncation mutants compared to wild-type Ii. Note that to generate Ii^97-Stop, the transmembrane segment of CTLA4 was fused to the C-terminus of Ii to maintain membrane tethering. (b) Schema depicting Ii truncation mutants compared to wild-type Ii when membrane embedded. Addition of CTLA4 transmembrane region converts Ii^97-Stop to a type I transmembrane protein, and consequently the CLIP sequence in each construct is situated on the same side of the ER membrane allowing access to class II as depicted. (c) K562 cells stably expressing the indicated class II allele were transiently transfected with wild-type Ii, Ii^ATG-120, Ii^97-Stop or empty vector (control). Lysates were then harvested from these cells, and non-reduced samples were immunoprecipitated and immunoblotted with the indicated mAbs. (d) K562 cells were stably transduced with the indicated combination of genes and then stained using anti-NGFR and anti-CLIP mAbs. The data shown are gated on ΔNGFR⁺ cells. Full, uncropped blots are provided in Supplementary Fig. 4.