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In vitro formation of ring-like structures by purified Drp1. (A) Electron micrograph of Drp1 incubated with 150 mM NaCl. (B) Drp1 incubated with GDP and AlF4−. Arrows point to the ring-like structures. (C) Examples of ring-like structures that were observed with GDP and AlF4−. The proteins were detected by negative staining with uranyl acetate.
