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. 2018 Mar 20;4:13. doi: 10.1038/s41421-018-0010-9

Fig. 2. TRIM29 regulates host immune response against viral infection in vivo.

Fig. 2

Real-time PCR assay a, c and ELISA b were used to detect the expression of cytokine, chemokine and antiviral protein in WT and Trim29−/− BMDMs stimulated with HSV-60, VACV-70, cGAMP, and DNA90 for 12 h. d WT and Trim29-/- mice (n = 8 per strain) were infected by intravenous injected HSV-1 (2 × 107 PFU per mouse) and monitored daily for 2 weeks for survival rate. e Viral titers of brains, livers and spleens from WT and Trim29−/− mice (n = 3 per strain) 2 and 4 days after HSV-1 infection. f ELISA analysis of IFN-α, IFN-β, TNF-α and IL-6 in sera from WT and Trim29−/− mice (n = 3 per strain) after HSV-1 infection. Data in all panels are representative of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 (Student’s t-test). Error bars are s.d.