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. 2018 Mar 20;4:13. doi: 10.1038/s41421-018-0010-9

Fig. 4. TRIM29 interacts with STING and mediates K48 linked ubiquitination and degradation of STING.

Fig. 4

a TRIM29-interacting proteins were identified by mass spectrum. Interaction between TRIM29 and STING was detected by co-immunoprecipitation (co-IP) in HSV-60 or cGMAP-stimulated BMDMs b and in the HEK 293T cell overexpressed TRIM29 and STING c. d Confocal microscopy of HEK 293T cells co-expressed Flag-TRIM29 and STING, non-treated and stimulated with HSV-60 for 6 h, followed by staining for Flag-TRIM29 (green) and STING (red). The bar in the picture stood for 1 μm. e HEK 293T cells were transfected with STING with or without TRIM29, treated with DMSO or MG132 for 12 h. STING and TRIM29 protein level was detected by IB. f HEK 293T cells were transfected with STING, TRIM29 and HA-tagged WT or mutant ubiquitin and subjected to ubiquitin assay. The ubiquitination of STING was detected by IB using anti-HA antibody. g WT and Trim29-/- macrophages were stimulated with HSV-60 for indicated time. The cells were lysed and subjected to IP with anti-STING antibody, the ubiquitination of STING was detected by IB using anti-ubiquitin and anti-K48 ubiquitin antibody. Data in all panels (except panel a) are representative of three independent experiments