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. 2018 Mar 19;8:4841. doi: 10.1038/s41598-018-23068-0

Figure 2.

Figure 2

HilD positively regulates the expression of grhD1 (SL1344_1872). (A) Expression of GrhD1-FLAG in the WT S. Typhimurium SL1344 strain and its derivative ∆hilD mutant containing or not the pK6-HilD plasmid, which expresses HilD from an arabinose-inducible promoter, or containing the pMPM-K6Ω vector. The expression of GrhD1-FLAG was analyzed from samples of bacterial cultures grown in LB at 37 °C by Western blotting, using monoclonal anti-FLAG antibodies. The expression of GroEL was also determined using polyclonal anti-GroEL antibodies, as a loading control. (B) Expression of the grhD1-cat transcriptional fusion contained in the pgrhD1-cat plasmid was determined in the WT S. Typhimurium strain and its derivative ∆hilD mutant carrying or not pK6-HilD or pMPM-K6Ω. Expression of HilD from pK6-HilD was activated by adding 0.001% L-arabinose to the medium. CAT specific activity was determined from samples of bacterial cultures grown in LB at 37 °C. Data are the average of three independent experiments done in duplicate. Bars represent the standard deviations. Statistically different values are indicated (***p < 0.001).