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. 2018 Mar 19;8:4802. doi: 10.1038/s41598-018-23037-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Overview of the proteomic strategies used to stratify cardiovascular risk in individuals aged between 30–50 years old. (B) Experimental design consisting of two different discovery phases, one of them using depleted plasma and the other analyzing non-depleted plasma from an independent cohort of patients. Moreover, a confirmation phase was performed employing two orthogonal techniques, selected reaction monitoring (SRM) and turbidimetry.