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. 2018 Mar 19;8:4829. doi: 10.1038/s41598-018-22862-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic real-time monitoring of proliferation and cytotoxicity using the xCELLigence system dedicated to adherent cell lines. Growth kinetics of human A549 lung carcinoma (A), A2780 ovarian carcinoma (B), SK-BR-3 breast adenocarcinoma (C) and MCF-7 breast adenocarcinoma (D) cells treated with scoulerine. Cells treated with 0.1% DMSO were used as vehicle control and 5% DMSO treated cells were used as positive control. The normalized cell index was measured over 72 h. Plots shown are representative of at least three replicate experiments in each case.