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. 2018 Mar 19;8:4829. doi: 10.1038/s41598-018-22862-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of scoulerine treatment on mitotic arrest. The Jurkat (A) and MOLT-4 (B) cells were exposed to scoulerine and 5 μM of nocodazole (an antineoplastic agent that disrupts microtubule function by binding to tubulin used as a reference compound). After 24 h, pSer10 histone H3-positive population (percentages are shown) was quantified by flow cytometry. The figure shows representative flow cytometry histograms depicting Ser10-phosphorylated histone H3 positive cells (gate) in cell cultures. Bars indicate mean ± SD, n = 3. *Significantly different to corresponding control (P ≤ 0.05).