Fig. 8.

Both the CRY1 pocket and tail are required to recapitulate the long period length of Cry1 rescues. a Models of chimeric CRYs used in the experiments in this figure. CRY1-like and CRY2-like pockets are shown in black and white, respectively. The first column shows WT CRY1 and CRY2; the second column shows CRYs with CRY1 tails and CRY2-like pockets; the final column shows CRYs with CRY2 tails and CRY1-like pockets. b Shows a period plot from experiments in which either the tail or pocket of CRY1 and CRY2 was exchanged for its paralog. Data are shown here as mean ± SEM (n = 9 plates per condition from three independent experiments. Asterisks show significance by unpaired t test with Welch’s correction compared to WT CRY1 (****p < 0.0001). Hashes denote significance by unpaired t test with Welch’s correction compared to WT CRY2 (^###p = 0.0006, ^####p < 0.0001). c Models depicting CRY chimeras in which both the pocket and tail have been exchanged for paralogous residues. d Rescue assays performed with WT CRY1, CRY2 7m C1T (CRY2 7m with CRY1 tail (res 498–606)), CRY1 7m C2T (CRY1 7m with CRY2 tail (res 516–593)), and WT CRY2 shown as mean ± SEM (n = 9 plates per condition from three independent experiments). e Period plot of the data shown in d. Mean ± SEM indicated by bars. Asterisks show significance by unpaired t test with Welch’s correction (****p < 0.0001)