Figure 2.

ZnAβ40 or ZnAβ42 assemble into oligomers and contain more exposed hydrophobic surfaces than ADDLs. (A,B) SV in AUC of ZnAβ or ADDLs consist of Aβ40 or Aβ42, respectively. The data were analyzed by a continuous c(s) distribution model and the sedimentation coefficients were calculated and shown. (C,D) Bis-ANS spectra of ZnAβ and ADDL. ZnAβ40, ZnAβ42, ADDL-Aβ40, and ADDL-Aβ42 at 100 µM were prepared. Before the measurement, all Aβ solution were diluted to 50 µM in 10 mM Tris-HCl, pH 7.4, then, mixed with 0.5 µM Bis-ANS dye. Bis-ANS fluorescence spectra were collected. The spectra were subtracted from the respective buffer control.