Figure 5.

EDTA-treated ZnAβ40 showed faster fibrillization rate and ZnAβ oligomers are unable to seed Aβ. (A,B) Fibrillization kinetics of (A) EDTA-treated ZnAβ40 or Aβ40 and (B) EDTA-treated ZnAβ42 or Aβ42 after seeding. Five % sonicated preformed fibril seeds were added into EDTA-treated ZnAβ and Aβ solution. Fibrillization kinetic was measured by ThT fluorescence. ZnAβ alone and buffer containing 5% fibril seeds were also monitored. (C,D) Fibrillization kinetics of Aβ40 (C) and Aβ42 (D) with or without Zn²⁺ after EDTA treatment. ZnAβ was first prepared and incubate for 1 day for oligomerization, then added EDTA to remove Zn²⁺ ion. Aβ alone solution was also treated with EDTA for comparison. Fibrillization kinetics was measured by ThT fluorescence. (E,F) Seeding experiment were performed by adding Aβ fibrils or ZnAβ40 (E) or ZnAβ42 (F) as seeds. The respective buffer were added to Aβ solution as negative control.