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. 2018 Mar 19;8:4827. doi: 10.1038/s41598-018-23218-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression profiling of the small intestine after dietary treatment with NTs for 14 days. (A) Venn diagram illustrations of genes found to be differentially expressed with or without NT treatment in the small intestine. The common genes from quanti-seq experiments were upregulated at least 2-fold (P < 0.05). (B) Quantitative expression analysis of genes that were highly expressed following NT treatment in the small intestine (n = 3). Real-time PCR analysis was conducted in triplicate and normalized to the expression levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Significant differences between control and treatment groups are indicated as ***P < 0.001, **P < 0.01, and *P < 0.05. Error bars indicate standard errors (SEs) of triplicate analyses.