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. 2018 Mar 19;8:4827. doi: 10.1038/s41598-018-23218-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of PI3K/Akt, ERK1/2, p38, and JAK/STAT signaling pathways in IPEC-J2 cells treated with or without NSs or TFF3 (n = 3). IPEC-J2 cells were treated with 100 μM NSs and 100 ng/µL TFF3 for 2 h, and the phosphorylated forms of Akt (Ser473), p38 (Thr180/Tyr182), ERK1/2 (Thr202/Tyr204), and JAK/STAT (Ser727) were detected by western blot analysis. PI3K/Akt (LY2944002), ERK1/2 (U0126), p38 (SB202190), and JAK/STAT (STA-12) were used as inhibitors of corresponding signaling pathways. Whole images of blots are presented in the supplemental information for Fig. 4.