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. Author manuscript; available in PMC: 2018 Mar 20.
Published in final edited form as: Biochim Biophys Acta. 2017 Jun 20;1863(9):2319–2332. doi: 10.1016/j.bbadis.2017.06.015

Fig. 5.

Fig. 5

Chromatin immunoprecipitation (ChIP) for detection of the interaction between the AR and a half ARE at the ADTRP promoter/regulatory region. (A) Schematic diagram of the promoter/regulatory region of the human ADTRP gene. TSS, the transcription start site (+ 1 position); ARE, androgen-responsive element. (B) ChIP analysis showing that a Myc-tagged AR interacts with the ARE at the proximal ADTRP promoter/regulatory region in HeLa cell. An anti-Myc antibody pulled down chromatin containing the ADTRP ARE, but not a non-specific, control distal genomic DNA fragment. (C) Positive control for ChIP in HeLa cells. An anti-RNA Polymerase II antibody pulled down the chromatin containing the ADTRP TSS, but not a non-specific, control distal genomic DNA fragment. (D) Similar ChIP analysis as in (B), but in EAhy926 endothelial cells. (E) Similar control ChIP analysis as in (C), but in EAhy926 endothelial cells. ***, P < 0.001 (n = 4); NS, not significant.