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. Author manuscript; available in PMC: 2018 Mar 20.
Published in final edited form as: Biochim Biophys Acta. 2017 Jun 20;1863(9):2319–2332. doi: 10.1016/j.bbadis.2017.06.015

Fig. 6.

Fig. 6

Androgen has similar effects on EC proliferation, cell cycle progression, migration and apoptosis as overexpression of ADTRP. (A) Cell proliferation analysis for EAhy926 cells treated with androgen vs. vehicle (no androgen) control. ***, P < 0.001 (n = 3). (B) Cell proliferation analysis for EAhy926 cells with overexpression of ADTRP (transfection with an expression plasmid for ADTRP) or without ADTRP overexpression (transfection with the empty vector). **, P < 0.01 (n = 3); ***, P < 0.001 (n =3). (C) Representative flow cytometry data showing the cell number at the S phase of cell cycle for EAhy926 cells with or without overexpression of ADTRP vs. vector, and treated with androgen vs. vehicle. (D) Summary data from (C). *, P < 0.05 (n = 3); **, P < 0.01 (n = 3). (E) Representative images for scratch-based cell migration analysis for EAhy926 cells with or without overexpression of ADTRP vs. vector, and treated with androgen vs. vehicle. (F) Summary data from (E). ***, P < 0.001 (n =3). (G) Representative flow cytometry images for analysis of apoptosis for EAhy926 cells with or without overexpression of ADTRP vs. vector, and treated with androgen vs. vehicle. (H) Summary data from experiments as shown in (G), ***, P < 0.001 (n = 3).