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. 2018 Mar 13;9:278. doi: 10.3389/fmicb.2018.00278

FIGURE 2.

FIGURE 2

Experimental assay and chemical structures of compounds used in this study. (A) Schematic representation of the experimental procedure. Jurkat-LTR-Luc [reporter cells for HTLV-1 productive infection in which expression of the luciferase gene is under the control of the viral Tax-dependent promoter (LTR)] are pretreated or not with drugs for 18 h before their co-culture with C91PL (HTLV-1 infected T-cell line) for 24 h. Cell viability and luciferase activity are then measured. (B) AZT (1) is used as the reference drug during all experiments, while 3TC (2) and d4T (3) are used as controls; the acyclic nucleoside phosphonate PMEA (4), its thio-derivative S-PMEA (6) and their prodrugs (8) and (9), respectively; the acyclic nucleoside phosphonate PMPA (5), its thio derivative S-PMPA (7) and their pro-drugs (10), (11), (12), and (13), respectively. Structures were drawn using ChemDraw. The molecule names (when available) are indicated under the structure.