FIGURE 3.

Control of cell viability during drug treatment. Jurkat-LTR-Luc reporter cells were treated for 42 h and the proportion of dead cells was determined by trypan blue dye exclusion test. Data were normalized to the proportion of dead cells in untreated control conditions, set to 1, to obtain a mortality index. A mortality index higher than 1 indicate a drug-induced mortality. Drugs were used in the following assays if the mortality index was below 1.5. Drug concentration and compound number are indicated. Results were grouped based on the drug family: (A) nucleoside analogs, (B) acyclic nucleoside phosphonates, (C) prodrugs of acyclic nucleoside phosphonates. No significant differences were observed (one-way ANOVA), n = 2–4 independent experiments with triplicate samples.