Fig. 4.

Cycloheximide chase experiments. a Western blot showing the protein levels of overexpressed NAA10-WT-V5 and NAA10-V111G-V5 before and 2, 4 and 6 h after protein synthesis was stopped by the addition of cycloheximide. b Quantification of NAA10-V5 bands from cycloheximide chase experiments. Western blots from 6 independent experiments were quantified using Imagelab 3.0 from Biorad, and the intensities in each band 2, 4 and 6 h after cycloheximide treatment were correlated both to the loading control (anti-β-tubulin) and to the intensity in the band representing NAA10-V5 before cycloheximide treatment. Each curve represents the average levels of NAA10 from 6 independent experiments combined. * statistically significant (p < 0.0005, student t-test)