Table 3.
RT-qPCR results and correlation. Values in parentheses represent Fold Changes (FC)
| Tissue | Gene name | Microarray | qPCR# | Correlation## | ||||
|---|---|---|---|---|---|---|---|---|
| H vs L | H vs M | M vs L | H vs L | H vs M | M vs L | |||
| Kidney | CYP27A1 | H < L (2.11) |
H < M (1.45) |
M < L (1.46) |
H < L*** (2.83) |
H < M** (1.60) |
M < L** (1.77) |
0.94 |
| NFATC2 | H < L (1.33) |
H > M (1.13) |
M < L** (1.51) |
H < L* (1.76) |
H > M (1.04) |
M < L* (1.82) |
0.66 | |
| PTH1R | H > L (1.14) |
H < M (1.09) |
M > L (1.26) |
H > L (1.03) |
H < M (1.20) |
M > L (1.24) |
0.62 | |
| SLC34A3 | H > L (1.04) |
H < M (1.26) |
M > L (1.31) |
H < L (1.30) |
H < M* (1.40) |
M > L (1.07) |
0.61 | |
| SPP1 | H < L (1.48) |
H > M (1.46) |
M < L*** (2.16) |
H < L* (2.02) |
H > M (1.19) |
M < L** (2.41) |
0.92 | |
| VDR | H > L*** (1.75) |
H < M (1.05) |
M > L*** (1.84) |
H > L*** (1.75) |
H < M (1.08) |
M > L*** (1.88) |
0.76 | |
| Jejunum | CYP24A1 | H < L*** (7.69) |
H < M (1.48) |
M < L*** (5.19) |
H < L** (222.14) |
H < M (2.79) |
M < L* (79.59) |
0.89 |
| C1QC | H > L** (1.70) |
H < M (1.18) |
M > L*** (2.02) |
H > L** (1.79) |
H < M (1.05) |
M > L** (1.88) |
0.83 | |
| C1R | H > L** (1.85) |
H < M (1.19) |
M > L*** (2.24) |
H > L** (2.19) |
H < M (1.15) |
M > L** (2.52) |
0.93 | |
| C1S | H > L*** (3.08) |
H < M (1.19) |
M > L*** (4.36) |
H > L** (2.48) |
H < M (1.34) |
M > L*** (3.33) |
0.89 | |
| C7 | H > L (2.02) |
H < M (1.18) |
M > L** (2.47) |
H > L* (2.64) |
H < M (1.14) |
M > L** (3.00) |
0.94 | |
| MMP2 | H > L*** (2.20) |
H > M (1.18) |
M > L** (1.88) |
H > L** (4.05) |
H > M (1.21) |
M > L** (3.35) |
0.92 | |
Significance level was set at ***p < 0.001; **p < 0.01, *p < 0.05 and q < 0.2 for results from microarray and qPCR analyses. Fold Changes are stated in parentheses. # - Values were calculated by factorial normalization on RPL32 expression values. ## - Correlation of normalized values was calculated by Spearman; the significance level for correlation was set at p < 0.01. Scatterplots of jejunal and renal transcripts are displayed in Additional file 6