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. 2018 Mar 20;18:41. doi: 10.1186/s12935-018-0540-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — miR-200c mimics inhibited clone formation and self-renewal capacities in cancer stem cell-like side population cells. a qPCR assays were performed to assess the available 5637 and T24 bladder cancer stem cell-like side population cells transfected with miR-200c mimics and negative control (NC). b Cell clone formation assays demonstrated that the clone formation ability of 5637 and T24 cells was significantly decreased in the miR-200c mimics group compared to the NC group. c The self-renewal capacity of bladder cancer stem cell-like side population cells (magnification, 100×). d miR-200c mimics affected the expression of EMT-associated factors such as E-cadherin, vimentin and ZEB1 and ZEB2 proteins. Data are presented as mean ± SD. **P < 0.01 vs. mimics NC group