Fig. 4.

XIST knockdown inhibited cell clone formation and self-renewal in cancer stem cell-like side population cells. a qPCR analysis was performed to detect the effective interference plasmids targeting XIST. b miR-200c was significantly increased in the XIST knockdown group compared to the control group. c The protein expression of BCSC markers was decreased after XIST interference tested by Western blotting. d The efficiency of clone formation was significantly decreased in the anti-XIST group compared to the anti-con group. e The self-renewal capacity of bladder cancer stem cell-like side population cells was dramatically decreased when cells were treated with anti-XIST than when treated with anti-con (magnification, 100×). f The protein expression levels of EMT-associated factors in bladder cancer stem cell-like side population cells detected by Western blotting. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 vs. anti-con group