Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 20;37:63. doi: 10.1186/s13046-018-0731-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 2 — Metformin induces G0/G1 cell cycle arrest, but not apoptosis in myeloma cell lines. a, b Representative results showing the distribution of cells in G0/G1, S, or G2 phase in RPMI8226 and U266 cells following treatment or with metformin (5 mM or 20 mM) or without for 24 and 48 h. Histograms showing the percentage of myeloma cells in G0/G1, S, and G2 phases. c Western blot showing repression of the G1 phase-related cell cycle regulatory proteins cyclin D1 and activation of p21^CIP1 and p27^KIP1 . d Histograms showing the percentage of apoptotic RPMI8226 and U266 cells following treatment with metformin (0, 5, 20 mM) for 24 and 48 h, as detected by flow cytometry. All the experiments were performed in triplicated and repeated on at least three occasions. **P < 0.01 and ***P < 0.001 compared with the control group