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. 2018 Mar 20;13:20. doi: 10.1186/s13000-018-0697-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Stereological estimation of tumour stroma ratio. a The area with the highest stroma density (yellow frame) is subjectively selected at low magnification (2×). b At higher magnification (10×) a grid of 4 x (6 × 7) points is superimposed on the selected area. Points hitting either tumour cells or stroma are counted separately. Tumour cells must to be present at all borders of the visual field (north-east-south-west)