Fig. 1.
H06 epialleles. a Genomic location of H06, sRNAs in M82 and IL8–3 whole seedlings and methylation in seedling leaves. The H06IL epiallele in IL8–3 was methylated in the CG, CHG and CHH contexts and the source of abundant sRNAs. Neither DNA methylation nor sRNAs were detected in the H06M82 epiallele. b High sRNA production at H06 in seedlings of three introgression lines, the Micro-Tom cultivar and the wild relative S. pimpinellifolium. c H06 methylation in leaves of M82, S. pennellii, IL8–3, Micro-Tom and S. pimpinellifolium determined by Sanger bisulfite sequencing (at least 10 independent clones per genotype). Primer sequences are given in Additional file 10, and H06 sequences in Additional file 11