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. 2018 Mar 20;19:203. doi: 10.1186/s12864-018-4590-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Virus-induced paramutation. a Tobacco Rattle Virus modified to contain a 500-bp H06 fragment. b DNA methylation in the progeny of the V₀ infected plant that gave methylated offspring, assessed by McrBC (n = 59 V₁ plants). V₁ methylation patterns were stable in the V₂ generation (n = 4, 3, 4 and 4 for V2_1 to V2_4), and exhibited weak paramutagenicity in backcrosses to M82 (V₁BC₁, n = 10 for both V1_3 × M82 and V1_4 × M82). c H06 methylation of a V₁ plant assessed by Sanger bisulfite sequencing (9 independent clones)