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ZEA-induced apoptosis in porcine granulosa cells. (A) Porcine granulosa cells were cultured in the presence of ZEA (10 μM and 30 μM) for 24 h or 72 h and stained with Annexin V and Propidium. Cell apoptosis levels were determined using flow cytometry. (B) the apoptotic and necrotic percentages of porcine granulosa cells were analyzed acording to the results of flow cytometry. All experiments were repeated at least three times.
