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. 2018 Feb 12;14(3):294–305. doi: 10.7150/ijbs.23898

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Immunostaining of RAD51 indicating DSBs in porcine granulosa cells. (A) Porcine granulosa cells were treated with ZEA at 10 μM and 30 μM for 24 h and stained by indirect immunofluorescence. (B) The result of immunostaining for RAD51 was observed using fluorescence microscopy. The number of positive cells with CY3- RAD51 was displayed as a histogram. (C) The fluorescence intensity of RAD51 positive cells was analyzed using Image J. Compared to the control group, * indicated significant differences (P < 0.05), ** indicated extremely significant differences (P < 0.01). All experiments were repeated at least three times.