Fig. 9.

Antisense transcription and novel splicing of HHV-6B U83 gene. Nearly all of the strand-specific RNA-seq reads from Sup-T1 cells at the annotated HHV-6B Z29 U83 gene were antisense to the existing annotation and included a novel splice site. The same splice site in the context of antisense transcript predominance was recovered from virus cultured in MOLT3 cells. No high-confidence peptides were recovered to this alternatively spliced antisense transcript by shotgun proteomics