Table 1.

Cell culture media preparations

Name	Base media	Serum	Additives
MCF-12A/MCF-10A	DMEM/F12, 1:1	5% equine	20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 0.1 μg/mL cholera toxin, 10 μg/mL insulin
MCF7	DMEM	5% fetal bovine
Rinse	DMEM/F12, 1:1, phenol red-free
CD-FBS	DMEM/F12, 1:1, phenol red-free	5% charcoal–dextran stripped fetal bovine
CD-ES	DMEM/F12, 1:1, phenol red-free	5% charcoal–dextran stripped equine	20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 0.1 μg/mL cholera toxin, 10 μg/mL insulin