Table 2.

Primers sequences used in direct qMSP

MiRNA gene	Methylated primers	Unmethylated primers	Temperature (°C)		Amplicon size (bp)
			M	U	M	U
miRNA-122	F:5′-TTGTTTTGAAAATTATTTTTTTGTTC-3′	F:5′-TTTTGAAAATTATTTTTTTGTTTGA-3′	57.2	57.3	122	120
	R:5′-CATCTACTCACCTAATCCACGAT-3′	R:5′-CCATCTACTCACCTAATCCACAAT-3′	56.5	58.5
miRNA-125b	F:5′-CGGTTAAAGTATAAATTATAGAGTTACGG-3′	F:5′-TGGTTAAAGTATAAATTATAGAGTTATGG-3′	57.8	57.3	181	179
	R:5′-ACTAACTACAAAACTTCCAAAAACG-3′	R:5′-TAACTACAAAACTTCCAAAAACACC-3′	54.7	56.9
miRNA-106b	F:5’-TCGGAAATTTATTTGGAAGTTTATC-3’	F:5′-TGGAAATTTATTTGGAAGTTTATTG-3’	59.2	53.9	105	105
	R:5’-GACAATCTACTTCAACTCCTCGAC-3’	R:5’-CAACAATCTACTTCAACTCCTCAAC-3’	57.2	57.7