Table 2.
Tick-borne pathogens from the six positive cats and their similarity with sequences deposited in the GenBank database
| Primer | Closest GenBank accession | Percent identity (no. of cats)a | Agent | Sample ID | New GenBank accessions |
|---|---|---|---|---|---|
| E.c 16S-fwd/E.c 16S-rev | KX987326 | 100 (1) | Ehrlichia canis | 017 | na |
| KX987326 | 100 (1) | Ehrlichia canis | 053 | na | |
| KX987326 | 99 (1b) | Ehrlichia canis | 002 | na | |
| E.c 16S-fwd/E.c 16S-rev + EHR16SD/EHR16SR | KY425447 | 99 (1) | Anaplasma bovis | 026 | MG431981 |
| Piroplasmid-F/Piroplasmid-R | KY649442 | 100 (1) | Hepatozoon felis | 063 | MG386484 |
| KY649443 | 100 (1) | Hepatozoon felis | 056 | MG386483 | |
| KY649443 | 99 (1b) | Hepatozoon felis | 002 | MG386482 |
Abbreviations: na not available, as sequences < 200 bp cannot be deposited in GenBank (see also Additional file 1: Table S1)
aOnly sequence identity ≥ 97% was considered as positive
bSame animal