Table 1.
Results of IFAT for R. conorii, E. canis, A. phagocytophilum and B. henselae antigens and PCR for E. canis, Anaplasma spp., Hepatozoon spp., Babesia spp. and filarioids in dogs with clinical leishmaniosis and healthy dogs. A comparison of the groups was made with Fisher’s exact test
| Assays | Number (%, 95% CI) of seroreactive dogs | |||
|---|---|---|---|---|
| IFAT | Dogs with clinical leishmaniosis (n = 61) |
Healthy dogs (n = 14) |
Total dogs (n = 75) |
P-value |
| R. conorii | 46 (75, 65–86) | 6 (43, 17–69) | 52 (69, 59–80) | 0.025* |
| E. canis | 34 (56, 43–68) | 9 (64, 39–89) | 43 (57, 46–69) | 0.766 |
| A. phagocytophilum | 32 (53, 40–65) | 1 (7, 0–21) | 33 (44, 33–55) | 0.002* |
| B. henselae | 25 (40, 28–52) | 3 (21, 0–43) | 28 (37, 26–48) | 0.147 |
| PCR | Dogs with clinical leishmaniosis (n = 60) |
Healthy dogs (n = 16) |
Total dogs (n = 76) |
P-value |
| Ehrlichia/Anaplasma spp. | 8 (13, 5–22) | 0 (0) | 8 (11, 4–17)a | 0.191 |
| H. canis and Babesia spp. | 1 (2, 0–5) | 0 (0) | 1 (1, 0–4)b | 0.606 |
| Filarioid DNA | 0 (0) | 0 (0) | 0 (0) | – |
Abbreviations: CI confidence interval, IFAT immunofluorescence antibody test, PCR polymerase chain reaction
aTwo dogs remained with a positive result for Anaplasma platys after performing a conventional PCR and sequencing
bOne dog was detected with Hepatozoon canis infection. No Babesia spp. or filarioid DNA were detected
*P-values statistically significant