Table 5.
Dogs positive by the different PCRs performed and the corresponding IFAT results for different antigens studied
| Dog ID |
qPCR L. infantum (parasite/ml); ELISA dilution (EU) |
IFAT | qPCR Ehrlichia/ Anaplasma |
Conventional PCR Ehrlichia/ Anaplasma |
PCR Hepatozoon/ Babesia |
Clinical signs and laboratory abnormalities | |||
|---|---|---|---|---|---|---|---|---|---|
| Rc | Ec | Ap | Bh | ||||||
| HCV-7 | Negative (0); Positive (195) |
512 | 1024 | 1024 | 256 | Positive | Negative | Negative | Moderate lymphadenomegaly, skin lesions. Thrombocytopenia, hypercholesterolemia, hyperproteinemia, hyperbetaglobulinemia |
| HCV-9 | Positive (51); Positive (39892) |
< 64 | < 64 | < 64 | 256 | Positive | Negative | Negative | Fever, weight loss, moderate lymphadenomegaly, ocular lesions. Pancytopenia, hypoalbuminemia, hypergammaglobulinemia |
| HCV-11 | Positive (4); Positive (12568) |
> 512 | < 64 | 64 | 128 | Positive | Negative | Negative | Skin lesions. Hypoalbuminemia, hypergammaglobulinemia, proteinuria |
| HCV-28 | Positive (807); Positive (30827) |
512 | 1024 | 256 | < 64 | Positive | Negative | Negative | Weight loss, moderate lymphadenomegaly, skin and ocular lesions. Regenerative anemia, hypoalbuminemia, hypergammaglobulinemia, proteinuria |
| HCV-31 | Positive (8); Positive (37360) |
64 | 64 | 128 | 64 | Positive | Negative | Negative | Fever, mild lymphadenomegaly, epistaxis. Anemia, lymphocytosis, thrombocytopenia, hypoalbuminemia, hypergammaglobulinemia |
| MO-1 | Positive (33); Positive (47) |
512 | 128 | 64 | < 64 | Positive | Positivea | Negative | Skin lesions. Hypercholesterolemia |
| MO-4 | Negative (0); Positive (188) |
256 | 64 | 1024 | < 64 | Negative | – | Positivea | Skin lesions. Mild hyperbetaglobulinemia |
| MED-5 | Negative (0); Positive (7170) |
64 | < 64 | < 64 | 256 | Positive | Positivea | Negative | Weight loss, moderate lymphadenomegaly, skin and ocular lesions, splenomegaly. Anemia, hypoalbuminemia |
| MED-7 | Negative (0); Positive (82) |
< 64 | < 64 | 1024 | < 64 | Positive | Negative | Negative | Moderate lymphadenomegaly, skin lesions. Hypoalbuminemia |
Abbreviations: Ap Anaplasma phagocytophilum, Bh Bartonella henselae, Ec Ehrlichia canis, IFAT immunofluorescence antibody test, PCR polymerase chain reaction, Rc Rickettsia conorii, qPCR real-time PCR
aThree pathogens were sequenced with the following results: two A. platys and one H. canis