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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cancer Res. 2017 Mar 29;15(7):842–851. doi: 10.1158/1541-7786.MCR-16-0387

Figure 2. Effects of DNA damage on quiescent prostate fibroblast gene expression, a.

Figure 2

Cellular DNA damage response (DDR) foci were determined by counting H2AX foci in PSC27 prostate fibroblasts that were proliferating (Pro) or quiescent by serum starvation (QSS) or quiescent by contact inhibition (QCI).

b. Immunofluorescence detection of H2AX foci (pink; arrow) in PSC27 prostate fibroblasts. P-CON, proliferating cells sham irradiated; P-RAD, proliferating cells following irradiation; Q-SS-RAD, serum-starved quiescent cells following irradiation; Q-CI-RAD, contact inhibited quiescent cells following irradiation. Nuclei were counterstained with DAPI (blue).

c. Gene expression profiles of quiescent PSC27 prostate fibroblasts before and after exposure to ionizing radiation. Shown are heatmaps of the subset of genes altered by 3-fold or greater with an expanded view of a subset of transcripts encoding secreted proteins.

d. Gene set enrichment analysis comparing transcript alterations in irradiated quiescent PSC27 prostate fibroblasts to previously reported gene expression alterations in proliferating fibroblasts following DNA damage(27).

e. Transcript quantitation by qRT-PCR of gene expression changes following ionizing radiation. C, proliferating PSC27 cells sham irradiated; Q, quiescent PSC27 cells sham irradiated; Q+R, quiescent PSC27 irradiated; R, proliferating PSC27 cells irradiated; Q+M, quiescent PSC27 cells treated with mitoxantrone; M, proliferating PSC27 cells treated with mitoxantrone.