Table 2.
Effect of the comt 17 mutation on the formation of Golgi stacks. org refers to the larval clusters or the Golgi when clearly identifiable. Results are expressed as ±SD.
| % of org membrane in
|
||||||
|---|---|---|---|---|---|---|
| Sorg/Vcyt* (μm−1) | Vesicular profiles (%) | Tubules (%) | Total cisternae (%) | Stacked cisternae/total cisternae | ||
| WT | Before incubation | 1.91 ± 0.20 | 36.0 ± 8.6 | 57.1 ± 7.8 | 7.0 ± 3.5 | nd |
| After 2-h incubation at 21°C in vivo | 2.04 ± 0.21 | 32.4 ± 9.3 | 42.5 ± 6.5 | 10.1 ± 3.9 | nd | |
| After 2-h incubation at 37°C in vivo | 3.09 ± 0.31 | 20.5 ± 4.9 | 34.1 ± 5.7 | 45.0 ± 8.2 | 0.45 ± 0.12 | |
| comt 17 | After 2-h incubation at 37°C in vivo | 3.64 ± 0.27 | 34.7 ± 6.8 | 49.3 ± 6.7 | 16.8 ± 9.7 | nd |
| After 2-h incubation at 37°C in vitro | 3.65 ± 0.30 | 38.6 ± 5.9 | 45.8 ± 7.4 | 15.2 ± 8.6 | nd | |
| comt 17/Y; TM6/+ | After 2-h incubation at 37°C in vitro | 4.13 ± 0.62 | 35.1 ± 6.8 | 50.1 ± 10.3 | 14.8 ± 7.3 | nd |
| comt 17/Y; HSN TM3, sb/+ | After 2-h incubation at 37°C in vitro | 6.99 ± 0.73 | 14.3 ± 7.5 | 32.7 ± 8.6 | 53.0 ± 5.4 | 0.64 ± 0.1 |
nd, not determined.
*
See MATERIALS AND METHODS for details.