Table 3.
Stereological analysis of the effect of ecdysone on the formation of the Golgi stacks. org refers to the larval clusters or the Golgi are when clearly identifiable. Results are expressed as ±SD.
| % of org membrane in
|
||||||
|---|---|---|---|---|---|---|
| Sorg/Vcyt* (μm−1) | Vesicular profiles | Tubules | Total cisternae | Stacked cisternae/total cisternae | ||
| WT in vitro | −ecd | 2.04 ± 0.42 | 43.5 ± 16.6 | 44.0 ± 13.1 | 11.5 ± 5.0 | nd |
| +ecd | 5.42 ± 1.13 | 13.8 ± 5.5 | 26.9 ± 7.9 | 58.8 ± 10.3 | 0.61 ± 0.10 | |
| Ecd1ts in vivo | at 29°C | 2.86 ± 0.74 | 28.5 ± 9.7 | 65 ± 14 | 7.0 ± 3.6 | nd |
| at 21°C | 3.31 ± 0.50 | 16.1 ± 5.6 | 23.1 ± 10 | 60.9 ± 15.0 | 0.72 ± 0.24 | |
| Ecd1ts in vitro | −ecd | 3.24 ± 0.43 | 32.1 ± 10 | 63.0 ± 15 | 4.6 ± 4 | nd |
| +ecd | 2.86 ± 0.20 | 22.6 ± 3.5 | 29.1 ± 8.2 | 47.7 ± 7 | 0.63 ± 0.25 | |
nd, not determined.
*
See MATERIALS AND METHODS for details.