Figure 3.

Effect of sulforaphane (SFN) treatment on cardiomyocyte apoptosis. The cardiomyocyte apoptosis levels of the Sham group (A), chronic heart failure (CHF) group (B) and the CHF +S FN group (C) were detected by using the terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay. (D) The apoptotic index was presented as the ratio of apoptotic nuclei (brown-stained) to total nuclei counted. (E) Bcl-2 and caspase-3 protein expression of different experimental groups were detected by Western blot. * P<0.05 versus sham operation group, ^# P<0.05 versus chronic heart failure (CHF) group.