Figure 5.

Analysis of binding partners of PSF. HL-60 cells were incubated with actinomycin D for 5 h when more than 50% of the cells become apoptotic. Lysates from apoptotic (Apo) and nonapoptotic (−) cells were immunoprecipitated with an anti-PTB antibody and Western blotted with the B92 antibody (A), immunoprecipitaed with anti–U1–70K and blotted with B92 (B), immunoprecipitated with anti–U1–70K and blotted with anti-SR (C), or immunoprecipitated with the B92 antibody and Western blotted with anti-SR antibodies (D).