Fig 4.

Effect of DEX on gene expression +/- leptin antagonist. A. Osteocalcin gene expression was significantly down-regulated by 10 and 100 nM DEX (*p=0.03, **p=0.004, respectively). B. The presence of the leptin antagonist (A500, 500 ng/mL) showed partial recovery of osteocalcin down regulation induced by DEX (100 nM) (**p=0.003 vs. 0, #p=0.035 vs. DEX 100). Upper panel, a representative gel; lower panel, semi-quantitative data. C. Osteocalcin protein expression was also significantly down-regulated by 10 and 100 nM DEX (*p<0.05). (n=6)