Fig 5.

Animal study in vitro. A. Inhibition of osteocalcin gene expression by DEX in primary osteoblastic cells of wild-type and db/db mice. Cells were treated with 100 nM of DEX for 24 h. Osteocalcin gene expression showed significant decrease in cells from wild-type mice, but not in db/db mice. B. In cells from wild-type mice, the post-receptor signalling molecule was associated with phophorylation of JAK2 in both DEX and leptin (Lep 20, leptin 20 ng/mL) treatment. In cells from db/db mice, the tendency of JAK2 phosphorylation was not significant. The data expressed as mean ± SD. * denotes P < 0.05 as compared to control cultures without DEX treatment (n=3).